ABL2 Polyklonaler Antikörper

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• KD/KO Validated

ABL2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 17693-1-AP

Synonyme

Abelson tyrosine-protein kinase 2, Abelson-related gene protein, ABLL, ARG, EC:2.7.10.2

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  WB analysis using 17693-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17693-1-AP (ABL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17693-1-AP

  IP result of anti-ABL2 (IP:17693-1-AP, 4ug; Detection:17693-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human gliomas using 17693-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17693-1-AP (ABL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 17693-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17693-1-AP (ABL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

17693-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA ABL2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABL2 fusion protein Ag11860
• Vollständiger Name: v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)
• Berechnetes Molekulargewicht: 1167 aa, 127 kDa
• Beobachtetes Molekulargewicht: 128-140 kDa
• GenBank-Zugangsnummer: BC065912
• Gene symbol: ABL2
• Gene ID (NCBI): 27
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABL2(Abelson tyrosine-protein kinase 2) is also named as ABLL, ARG and belongs to the protein kinase superfamily. It is a membrane-anchored nonreceptor tyrosine kinase that is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity and it has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. ABL2 can be polyubiquitinated and the polyubiquitination of ABL2 leads to degradation. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 114 kDa to 128 kDa. The antibody is specific to the others except the 60 kDa isoform of ABL2.

Publikationen

Species	Application	Title
rat	IF	Bioact Mater; High proportion strontium-doped micro-arc oxidation coatings enhance early osseointegration of titanium in osteoporosis by anti-oxidative stress pathway.; Authors - Xinkun Shen
rat	WB	Theranostics; Non-invasive photobiomodulation treatment in an Alzheimer Disease-like transgenic rat model.; Authors - Luodan Yang
human	WB	Elife; Abelson tyrosine-protein kinase 2 regulates myoblast proliferation and controls muscle fiber length.; Authors - Jennifer K Lee ; KO Validated
human	WB,IHC,CoIP	Oncogene; Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement; Authors - Ze Zhang
human	WB	Genomics; Bone marrow mesenchymal stem cells-derived exosomal microRNA-19b-1-5p reduces proliferation and raises apoptosis of bladder cancer cells via targeting ABL2.; Authors - Dewang Fu
human	WB	Biochim Biophys Acta; Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration.; Authors - Siddhesh Aras