ACAA2 Polyklonaler Antikörper

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• KD/KO Validated

ACAA2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 11111-1-AP

Synonyme

Beta ketothiolase, Acyl-CoA hydrolase, mitochondrial, Acetyl-CoA acyltransferase, Acetyl-CoA acetyltransferase, Acetyl CoA acyltransferase

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  WB analysis using 11111-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11111-1-AP (ACAA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11111-1-AP

  WB result of ACAA2 antibody (11111-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAA2 transfected HEK-293 cells.

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  IHC staining of human lung cancer using 11111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11111-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11111-1-AP (ACAA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11111-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11111-1-AP (ACAA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse liver using 11111-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11111-1-AP (ACAA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolongewebe, humanes Lebergewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 2 publications below
• IP: See 2 publications below

Produktinformation

11111-1-AP bindet in WB, IHC, IP, ELISA ACAA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACAA2 fusion protein Ag1609
• Vollständiger Name: acetyl-Coenzyme A acyltransferase 2
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC001918
• Gene symbol: ACAA2
• Gene ID (NCBI): 10449
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Oncotarget; Genomic binding and regulation of gene expression by the thyroid carcinoma-associated PAX8-PPARG fusion protein.; Authors - Yanxiao Zhang
rat	WB	EBioMedicine; PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.; Authors - Zhongshi Lyu
rat	WB,IHC	PLoS One; Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats.; Authors - Baandrup Jonas D JD
sheep	WB	Front Genet; Identification of miRNA in Sheep Intramuscular Fat and the Role of miR-193a-5p in Proliferation and Differentiation of 3T3-L1.; Authors - Fuhui Han
rat	WB	Gerontology; Dietary Restriction Increases Protein Acetylation in the Livers of Aged Rats.; Authors - Nakamura Akihiro A
human	WB	J Biol Chem; CLPX regulates mitochondrial fatty acid β-oxidation in liver cells; Authors - Ko Suzuki