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ACC1 Polyklonaler Antikörper
ACC1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (3)
Anwendung
WB, IHC, IF/ICC, IP, RIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 21923-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Maushirngewebe, Rattenhirngewebe |
| Erfolgreiche IP | HepG2-Zellen |
| Erfolgreiche Detektion in IHC | Maus-Skelettmuskelgewebe, Maushirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:20000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 7 publications below |
| WB | See 167 publications below |
| IHC | See 15 publications below |
| IF | See 9 publications below |
| IP | See 2 publications below |
| RIP | See 1 publications below |
Produktinformation
21923-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA ACC1 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Maus, Ratte, Rind |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | ACC1 fusion protein Ag16452 |
| Vollständiger Name | acetyl-Coenzyme A carboxylase alpha |
| Berechnetes Molekulargewicht | 2383 aa, 275 kDa |
| Beobachtetes Molekulargewicht | 250 kDa |
| GenBank-Zugangsnummer | BC137287 |
| Gene symbol | Acetyl-CoA Carboxylase 1 |
| Gene ID (NCBI) | 31 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for ACC1 antibody 21923-1-AP | Protokoll herunterladen |
| IHC protocol for ACC1 antibody 21923-1-AP | Protokoll herunterladenl |
| IF protocol for ACC1 antibody 21923-1-AP | Protokoll herunterladen |
| IP protocol for ACC1 antibody 21923-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cancer Cell KMT2C deficiency drives transdifferentiation of double-negative prostate cancer and confer resistance to AR-targeted therapy | ||
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Signal Transduct Target Ther The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease | ||
Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. | ||
Adv Sci (Weinh) Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells | ||
Autophagy Buddleoside alleviates nonalcoholic steatohepatitis by targeting the AMPK-TFEB signaling pathway |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kishor (Verified Customer) (02-22-2019) | I have got some good results with this Ab, but the band are not at the exact place (molecular weight) where it supposed to be
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