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ACADL-Specific Polyklonaler Antikörper

ACADL-Specific Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17526-1-AP

Synonyme

ACAD4, ACADL, ACADL-Specific, LCAD



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausnierengewebe, Mausherzgewebe, Mauslebergewebe, Mauslungengewebe
Erfolgreiche IPMausnierengewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17526-1-AP bindet in WB, IP, IHC, IF, ELISA ACADL-Specific und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte, Mutterschafe
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name acyl-Coenzyme A dehydrogenase, long chain
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht 48 kDa
GenBank-ZugangsnummerNM_001608
Gene symbol ACADL
Gene ID (NCBI) 33
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.

Protokolle

Produktspezifische Protokolle
WB protocol for ACADL-Specific antibody 17526-1-APProtokoll herunterladen
IP protocol for ACADL-Specific antibody 17526-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
mouseWB

Circ Res

NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.

Authors - Dan Tong
humanWB,IF

Int J Biol Sci

Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma

Authors - Jingwei Cai
mouseWB

Diabetes

Pivotal Role of O-GlcNAc Modification in Cold-Induced Thermogenesis by Brown Adipose Tissue Through Mitochondrial Biogenesis.

Authors - Natsuko Ohashi
mouse, humanWB, IHC

Cell Rep

HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.

Authors - De Huang
  • KD Validated
mouseWB

Cell Mol Gastroenterol Hepatol

Fatty Acids Inhibit LAMP2-Mediated Autophagy Flux via Activating ER Stress Pathway in Alcohol-Related Liver Disease.

Authors - Wei Guo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wu (Verified Customer) (04-10-2021)

LCAD antibody worked good in IF assay at 1:50 dilution.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Liver
ACADL-Specific Antibody Immunofluorescence validation (1:50 dilution) in Liver (Cat no:17526-1-AP)
FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain