ACADM Monoklonaler Antikörper

ACADM Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1A11G6

Rezensionen (1)

Kat-Nr. : 67742-1-Ig

Synonyme

1A11G6, ACAD, ACAD 1, ACAD1, EC:1.3.8.7

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  WB analysis using 67742-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of rat liver using 67742-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67742-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67742-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67742-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 67742-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 67742-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 67742-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ACADM antibody (67742-1-Ig, Clone: 1A11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

67742-1-Ig bindet in WB, IHC, IF-P, ELISA ACADM und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ACADM fusion protein Ag30620
• Vollständiger Name: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• Berechnetes Molekulargewicht: 421 aa, 47 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC005377
• Gene symbol: ACADM
• Gene ID (NCBI): 34
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

Publikationen

Species	Application	Title
rat	WB	Int J Mol Sci; High-Dose Fenofibrate Stimulates Multiple Cellular Stress Pathways in the Kidney of Old Rats; Authors - Agata Wrońska
mouse	WB,IF	Phytomedicine; Shenqi Pill alleviates acetaminophen-induced liver injury: a comprehensive strategy of network pharmacology and spectrum-effect relationship reveals mechanisms and active components; Authors - Qihan Luo
human	WB	Adv Sci (Weinh); N-glycosylation Modification of CTSD Affects Liver Metastases in Colorectal Cancer; Authors - Nan Xiong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Agata (Verified Customer) (04-16-2024)

The antibody used in WB gave a single clear band, of appropriate weight, with a strong signal, in rat kidney tissue homogenates - the antibody was excellent on every try.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: rat kidney

ACADM Antibody Western Blot validation (1:5000 dilution) in rat kidney (Cat no:67742-1-Ig)