ACADM Monoklonaler Antikörper

ACADM Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A11G6

Kat-Nr. : 67742-1-PBS

Synonyme

1A11G6, ACAD, ACAD 1, ACAD1, EC:1.3.8.7

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  WB analysis using 67742-1-Ig (same clone as 67742-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67742-1-Ig (same clone as 67742-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67742-1-Ig (same clone as 67742-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ACADM antibody (67742-1-Ig, Clone: 1A11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67742-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA ACADM und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ACADM fusion protein Ag30620
• Vollständiger Name: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• Berechnetes Molekulargewicht: 421 aa, 47 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC005377
• Gene symbol: ACADM
• Gene ID (NCBI): 34
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.