ACADS Polyklonaler Antikörper

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• KD/KO Validated

ACADS Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 16623-1-AP

Synonyme

ACAD3, ACADS, Butyryl CoA dehydrogenase, SCAD

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Galerie der Validierungsdaten

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  WB analysis using 16623-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16623-1-AP (ACADS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 16623-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16623-1-AP (ACADS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16623-1-AP

  IP Result of anti-ACADS (IP:16623-1-AP, 4ug; Detection:16623-1-AP 1:500) with HEK-293 cells lysate 3000ug.

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  IHC staining of human kidney using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16623-1-AP (ACADS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16623-1-AP (ACADS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, COS-7-Zellen, Mauslebergewebe, Rattenlebergewebe, Rattennierengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

16623-1-AP bindet in WB, IP, IHC, IF, ELISA ACADS und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACADS fusion protein Ag9922
• Vollständiger Name: acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
• Berechnetes Molekulargewicht: 412 aa, 44 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC025963
• Gene symbol: ACADS
• Gene ID (NCBI): 35
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACADS is also named as SCAD(Short-chain specific acyl-CoA dehydrogenase, mitochondrial) and belongs to the acyl-CoA dehydrogenase family. It is a tetrameric mitochondrial flavoprotein. It is synthesized as a 44 kDa precursor, transported into mitochondria, and proteolytically processed to its 41 kDa mature form. Acyl-CoA de- hydrogenases (ACADs) are a family of flavoproteins that are involved in theβ-oxidation of the fatty acyl-CoA derivatives.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
human	WB	Cell Rep; HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.; Authors - De Huang
human	WB	Aging (Albany NY); ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas.; Authors - Diyu Chen ; KD Validated
mouse, rat	WB	Arch Toxicol; Protective effects of dioscin against alcohol-induced liver injury.; Authors - Xu Tingting T
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Dietary iron overload mitigates atherosclerosis in high-fat diet-fed apolipoprotein E knockout mice: Role of dysregulated hepatic fatty acid metabolism.; Authors - Lin Xiao