ACAP1 Polyklonaler Antikörper

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• KD/KO Validated

ACAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 20135-1-AP

Synonyme

Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1, Centaurin beta 1, Centaurin-beta-1, CENTB1, Cnt b1

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  WB analysis of Jurkat using 20135-1-AP

  WB result of ACAP1 antibody (20135-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells.

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  WB analysis using 20135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20135-1-AP (ACAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 20135-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20135-1-AP (ACAP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 20135-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20135-1-AP (ACAP1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of Jurkat using 20135-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ACAP1 antibody (20135-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

20135-1-AP bindet in WB, IHC, IF/ICC, ELISA ACAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACAP1 fusion protein Ag14068
• Vollständiger Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
• Berechnetes Molekulargewicht: 740 aa, 82 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC018543
• Gene symbol: ACAP1
• Gene ID (NCBI): 9744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

Publikationen

Species	Application	Title
human	WB	Comput Struct Biotechnol J; Integrated analysis and validation reveal ACAP1 as a novel prognostic biomarker associated with tumor immunity in lung adenocarcinoma; Authors - Ning Wang
human	WB,IF	Methods Cell Biol; An ACAP1 coat complex acting in endocytic recycling.; Authors - Jian Li