ACAP3 Polyklonaler Antikörper

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• KD/KO Validated

ACAP3 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17570-1-AP

Synonyme

KIAA1716, Cnt-b5, CENTB5, Centaurin-beta-5, Centaurin beta 5

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  WB analysis of Jurkat using 17570-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17570-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 17570-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 17570-1-AP (ACAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

17570-1-AP bindet in WB, IF/ICC, ELISA ACAP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACAP3 fusion protein Ag11777
• Vollständiger Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 3
• Berechnetes Molekulargewicht: 834 aa, 92 kDa
• Beobachtetes Molekulargewicht: 85kda, 92 kDa
• GenBank-Zugangsnummer: BC047001
• Gene symbol: ACAP3
• Gene ID (NCBI): 116983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACAP3 (Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 3) , also known as CENTB5, is a protein-coding gene that belongs to the ACAP family of GTPase-activating proteins (GAPs). ACAP3 is involved in neuronal migration in the developing cerebral cortex of mice. Knockdown of ACAP3 in the developing cortical neurons of mice in utero significantly abrogated neuronal migration in the cortical layer, ACAP3 is involved in neuronal migration in the developing cerebral cortex of mice. Knockdown of ACAP3 in the developing cortical neurons of mice in utero significantly abrogated neuronal migration in the cortical layer (PMID: 28919417),

Publikationen

Species	Application	Title
human,mouse	WB,IF	Biochem J; ACAP3 regulates neurite outgrowth through its GAP activity specific to Arf6 in mouse hippocampal neurons.; Authors - Yuki Miura
mouse	WB	Biochem Biophys Res Commun; ACAP3, the GTPase-activating protein specific to the small GTPase Arf6, regulates neuronal migration in the developing cerebral cortex.; Authors - Yuki Miura ; KD Validated
mouse	WB	Autophagy; Aging Differentially Affects Axonal Autophagosome Formation and Maturation; Authors - Heather Tsong