ACAT2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ACAT2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Rezensionen (1)

Kat-Nr. : 14755-1-AP

Synonyme

acetoacetyl CoA thiolase, Acetyl-CoA acetyltransferase, cytosolic, Acetyl-CoA transferase-like protein, ACTL, Cytosolic acetoacetyl-CoA thiolase

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  WB analysis of HepG2 using 14755-1-AP

  WB result of ACAT2 antibody (14755-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT2 transfected HepG2 cells.

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  WB analysis using 14755-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14755-1-AP (ACAT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14755-1-AP

  IP result of anti-ACAT2 (IP:14755-1-AP, 4ug; Detection:14755-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14755-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAT2 antibody (14755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 14755-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ACAT2 Polyclonal antibody (14755-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HepG2-Zellen, K-562-Zellen, MOLT-4-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Lungengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 3 publications below

Produktinformation

14755-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA ACAT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACAT2 fusion protein Ag6503
• Vollständiger Name: acetyl-Coenzyme A acetyltransferase 2
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 40-42 kDa
• GenBank-Zugangsnummer: BC000408
• Gene symbol: ACAT2
• Gene ID (NCBI): 39
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		Cell Res; Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.; Authors - Yanjing Guo
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
human	IHC	Cancer Cell Int; Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer; Authors - Enkui Zhang
mouse	WB	iScience; Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice.; Authors - George D Dalton
mouse	WB	J Agric Food Chem; Oral Administration of Succinoglycan Riclin Improves Diet-Induced Hypercholesterolemia in Mice.; Authors - Yunxia Yang
human	WB	Food Res Int; Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo.; Authors - Suwen Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah-Eve (Verified Customer) (03-01-2023)

Very good working on PVDF membrane, blocking solution milk 5%

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human pulmonary artery smooth muscle cells

ACAT2 Antibody Western Blot validation (1:2000 dilution) in Human pulmonary artery smooth muscle cells (Cat no:14755-1-AP)