ACBD5 Polyklonaler Antikörper

ACBD5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 21080-1-AP

Synonyme

KIAA1996, Acyl-CoA-binding domain-containing protein 5

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  WB analysis using 21080-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21080-1-AP (ACBD5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 21080-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21080-1-AP (ACBD5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 21080-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 21080-1-AP (ACBD5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 21080-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21080-1-AP (ACBD5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, L02-Zellen, MCF-7-Zellen, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

21080-1-AP bindet in WB, IHC, ELISA ACBD5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACBD5 fusion protein Ag15287
• Vollständiger Name: acyl-Coenzyme A binding domain containing 5
• Berechnetes Molekulargewicht: 534 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC030555
• Gene symbol: ACBD5
• Gene ID (NCBI): 91452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACBD5, also known as RDLKD. It is expected to be located in the peroxisome membrane, which is ubiquitinated in brain and kidney tissue. This gene encodes a member of the acyl-Coenzyme A binding protein family, known to function in the transport and distribution of long chain acyl-Coenzyme A in cells. This gene may play a role in the differentiation of megakaryocytes and formation of platelets. A related protein in yeast is involved in autophagy of peroxisomes. A mutation in this gene has been associated with autosomal dominant thrombocytopenia. The molecular weight of ACBD5 is 60 kDa.

Publikationen

Species	Application	Title
mouse	WB	J Biol Chem; Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.; Authors - Pamela A Young
mouse	WB	Mol Cell Biochem; Differential distribution of peroxisomal proteins points to specific roles of peroxisomes in the murine retina.; Authors - Yannick Das
human	WB	bioRxiv; The p97-UBXD8 complex maintains peroxisome abundance by suppressing pexophagy; Authors - Iris D Montes