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ACC1 Monoklonaler Antikörper
ACC1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human, Maus, Ratte und mehr (3)
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
CloneNo.
1A11G10
Kat-Nr. : 67373-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen |
| Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:50000 |
| Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 36 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Produktinformation
67373-1-Ig bindet in WB, IHC, IF/ICC, ELISA ACC1 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Affe, hamster, Maus, Ratte, Ziege |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | ACC1 fusion protein Ag17503 |
| Vollständiger Name | acetyl-Coenzyme A carboxylase alpha |
| Berechnetes Molekulargewicht | 2383 aa, 275 kDa |
| Beobachtetes Molekulargewicht | 250-270 kDa |
| GenBank-Zugangsnummer | BC137287 |
| Gene symbol | Acetyl-CoA Carboxylase 1 |
| Gene ID (NCBI) | 31 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for ACC1 antibody 67373-1-Ig | Protokoll herunterladen |
| IHC protocol for ACC1 antibody 67373-1-Ig | Protokoll herunterladenl |
| IF protocol for ACC1 antibody 67373-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy | ||
EMBO J Rab2A-mediated Golgi-lipid droplet interactions support very-low-density lipoprotein secretion in hepatocytes | ||
Theranostics Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription. | ||
Phytomedicine Garlic-derived exosomes regulate PFKFB3 expression to relieve liver dysfunction in high-fat diet-fed mice via macrophage-hepatocyte crosstalk |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Parijat (Verified Customer) (09-09-2023) | Goof antibody
|
FH Priya (Verified Customer) (06-21-2023) | Used this antibody for Caco2 cells and mice tissue
|





