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ACC1 Monoklonaler Antikörper

ACC1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(39)

CloneNo.

1A11G10

Kat-Nr. : 67373-1-Ig

Synonyme

ACACA, ACC-alpha, ACCA, ACC, ACAC



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:10000-1:50000
Immunhistochemie (IHC)IHC : 1:500-1:2000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

67373-1-Ig bindet in WB, IHC, IF/ICC, ELISA ACC1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, hamster, Maus, Ratte, Ziege
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen ACC1 fusion protein Ag17503
Vollständiger Name acetyl-Coenzyme A carboxylase alpha
Berechnetes Molekulargewicht 2383 aa, 275 kDa
Beobachtetes Molekulargewicht 250-270 kDa
GenBank-ZugangsnummerBC137287
Gene symbol Acetyl-CoA Carboxylase 1
Gene ID (NCBI) 31
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ACC1 antibody 67373-1-IgProtokoll herunterladen
IHC protocol for ACC1 antibody 67373-1-IgProtokoll herunterladenl
IF protocol for ACC1 antibody 67373-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu
humanWB

Autophagy

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang
mouseWB

EMBO J

Rab2A-mediated Golgi-lipid droplet interactions support very-low-density lipoprotein secretion in hepatocytes

Authors - Min Xu
humanWB

EMBO J

CARS senses cysteine deprivation to activate AMPK for cell survival.

Authors - Mengqiu Yuan
humanWB

Theranostics

Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription.

Authors - Gengyi Zou
mouseWB

Phytomedicine

Garlic-derived exosomes regulate PFKFB3 expression to relieve liver dysfunction in high-fat diet-fed mice via macrophage-hepatocyte crosstalk

Authors - Jinfan Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Parijat (Verified Customer) (09-09-2023)

Goof antibody

  • Applications: Immunofluorescence
FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells and mice tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Caco2, Mice colon