ACC1 Rekombinanter Antikörper

ACC1 Rekombinant Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2I13

Kat-Nr. : 81656-1-PBS

Synonyme

ACACA, ACC-alpha, ACCA, ACC, ACAC

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  WB analysis using 81656-1-RR (same clone as 81656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81656-1-RR (ACC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81656-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 81656-1-RR (same clone as 81656-1-PBS)

  IP result of anti-ACC1 (IP:81656-1-RR, 4ug; Detection:81656-1-RR 1:2000) with HepG2 cells lysate 1640 ug. This data was developed using the same antibody clone with 81656-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81656-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81656-1-RR against Human ACC1 were performed. The affinity constant is 2.28 nM.

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Geprüfte Anwendungen

Produktinformation

81656-1-PBS bindet in WB, IP, Indirect ELISA ACC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ACC1 fusion protein Ag16452
• Vollständiger Name: acetyl-Coenzyme A carboxylase alpha
• Berechnetes Molekulargewicht: 2383 aa, 275 kDa
• Beobachtetes Molekulargewicht: 250 kDa
• GenBank-Zugangsnummer: BC137287
• Gene symbol: Acetyl-CoA Carboxylase 1
• Gene ID (NCBI): 31
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.