ACE Polyklonaler Antikörper

ACE Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24743-1-PBS

Synonyme

DCP1, DCP, Angiotensin-converting enzyme, soluble form, Angiotensin-converting enzyme, Angiotensin converting enzyme

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  WB analysis of mouse heart using 24743-1-AP (same clone as 24743-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 24743-1-AP (ACE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

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  WB analysis of rat lung using 24743-1-AP (same clone as 24743-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 24743-1-AP (ACE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

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  IHC staining of rat lung using 24743-1-AP (same clone as 24743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 24743-1-AP (same clone as 24743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 24743-1-AP (same clone as 24743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 24743-1-AP (same clone as 24743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 24743-1-AP (same clone as 24743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24743-1-PBS bindet in WB, IHC, Indirect ELISA ACE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACE fusion protein Ag20654
• Vollständiger Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
• Berechnetes Molekulargewicht: 150 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC036375
• Gene symbol: ACE
• Gene ID (NCBI): 1636
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).