ACE2 Rekombinanter Antikörper

ACE2 Rekombinant Antikörper für IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4H12

Kat-Nr. : 80063-1-PBS

Synonyme

ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15

---

• 

  IHC staining of human kidney using 80063-1-RR (same clone as 80063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80063-1-RR (ACE2 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 80063-1-RR (same clone as 80063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80063-1-RR (ACE2 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80063-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 80063-1-RR (same clone as 80063-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (80063-1-RR, Clone: 4H12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80063-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

80063-1-PBS bindet in IHC, IF-P, Indirect ELISA ACE2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ACE2 fusion protein Ag30683
• Vollständiger Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Berechnetes Molekulargewicht: 805 aa, 92 kDa
• GenBank-Zugangsnummer: BC048094
• Gene symbol: ACE2
• Gene ID (NCBI): 59272
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088). 80063-1-RR is specific for ACE2, and its antigen region is 30-356aa.