Acetylcholinesterase Polyklonaler Antikörper

Acetylcholinesterase Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Rezensionen (2)

Kat-Nr. : 17975-1-AP

Synonyme

ACHE, YT, N ACHE, EC:3.1.1.7, ARACHE

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• 

  WB analysis of mouse liver using 17975-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17975-1-AP (Acetylcholinesterase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17975-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17975-1-AP (Acetylcholinesterase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 17975-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17975-1-AP (Acetylcholinesterase antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 17975-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17975-1-AP (Acetylcholinesterase antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below

Produktinformation

17975-1-AP bindet in WB, IHC, ELISA Acetylcholinesterase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Acetylcholinesterase fusion protein Ag12146
• Vollständiger Name: acetylcholinesterase (Yt blood group)
• Berechnetes Molekulargewicht: 614aa,68 kDa; 640aa,71 kDa
• Beobachtetes Molekulargewicht: 66-72 kDa
• GenBank-Zugangsnummer: BC094752
• Gene symbol: Acetylcholinesterase
• Gene ID (NCBI): 43
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The classical role of acetylcholinesterase (AChE) is to terminate cholinergic neurotransmission by rapid hydrolysis of the neurotransmitter acetylcholine (ACh) at cholinergic synapses and neuromuscular junctions(PMID:20054652). AChE activity is significantly lower in aspirates from squamous cell carcinomas and the molecular distribution of AChE in both bronchial cells and fluids consisted of amphiphilic monomers and dimers(PMID:18211261). It has 4 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
rat	WB	ACS Sens; Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish.; Authors - Jianlong Ma
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
mouse	WB	Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo
mouse	IHC	Front Pharmacol; Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice.; Authors - Yang Yang
		J Alzheimers Dis; Lithium Attenuates Scopolamine-Induced Memory Deficits with Inhibition of GSK-3β and Preservation of Postsynaptic Components.; Authors - Wu Yuan-Yuan YY

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zulaikha (Verified Customer) (09-21-2022)

Consistent and reproducible results.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

FH

Dr Shane (Verified Customer) (08-17-2020)

Very good antibody and worked ok.

• Applications: Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Cell culture
• Cell Tissue Type: Chemical combination