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ACIN1 Polyklonaler Antikörper

ACIN1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23937-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using ACIN1 Polyclonal antibody (23937-1-AP)

    WB analysis using 23937-1-AP (same clone as 23937-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23937-1-AP (ACIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23937-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ACIN1 Polyclonal antibody (23937-1-AP)

    IHC staining of mouse cerebellum using 23937-1-AP (same clone as 23937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23937-1-AP (ACIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23937-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

23937-1-PBS bindet in WB, IHC, Indirect ELISA ACIN1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ACIN1 fusion protein Ag21036
Vollständiger Name apoptotic chromatin condensation inducer 1
Berechnetes Molekulargewicht 1341 aa, 152 kDa
Beobachtetes Molekulargewicht250 kDa, 80-85 kDa
GenBank-ZugangsnummerBC140805
Gene symbol ACIN1
Gene ID (NCBI) 22985
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. ACIN1 is a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. There are three isoforms of human acinus (the L, S and S' forms) shows at about 80-85 kDa and 220-250 kDa by Western Blot, which were probably generated by alternative splicing (PMID: 10490026). 23937-1-AP can detect all three isoforms of ACIN1. Another antibody 30522-1-AP specificly targets at the ACIN1-L.