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ACIN1-L specific Polyklonaler Antikörper

ACIN1-L specific Polyklonal Antikörper für WB, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30522-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using ACIN1-L specific Polyclonal antibody (30522-1-AP)

    WB analysis using 30522-1-AP (same clone as 30522-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30522-1-AP (ACIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30522-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using ACIN1-L specific Polyclonal antibody (30522-1-AP)

    IF Staining of mouse cerebellum using 30522-1-AP (same clone as 30522-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ACIN1-L specific antibody (30522-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30522-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30522-1-PBS bindet in WB, IF-P, Indirect ELISA ACIN1-L specific und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ACIN1-L specific fusion protein Ag30506
Vollständiger Name apoptotic chromatin condensation inducer 1
Berechnetes Molekulargewicht 1341 aa, 152 kDa
Beobachtetes Molekulargewicht240 kDa
GenBank-ZugangsnummerBC140805
Gene symbol ACIN1
Gene ID (NCBI) 22985
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. ACIN1 is a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. There are three isoforms of human acinus (the L, S and S' forms) shows at about 80-85 kDa and 220-250 kDa by Western Blot, which were probably generated by alternative splicing (PMID: 10490026). 30522-1-AP specificly targets at the ACIN1-L, while another antibody 23937-1-AP can detect all three isoforms of ACIN1.