ACLY Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ACLY Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(93)

Rezensionen (2)

Kat-Nr. : 15421-1-AP

Synonyme

ATP citrate lyase, ACL, ATP-citrate, ATP-citrate synthase, EC:2.3.3.8

---

• 

  WB analysis of A549 using 15421-1-AP

  WB result of ACLY antibody (15421-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACLY transfected A549 cells.

• 

  WB analysis using 15421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ACLY antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15421-1-AP

  IP result of anti-ACLY (IP:15421-1-AP, 4ug; Detection:15421-1-AP 1:8000) with HeLa cells lysate 1320 ug.

• 

  IHC staining of human prostate cancer using 15421-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15421-1-AP (ACLY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15421-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15421-1-AP (ACLY antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 15421-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACLY antibody (15421-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HepG2 using 15421-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ACLY (15421-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, L02-Zellen, MCF-7-Zellen, Maushirngewebe, Mauslebergewebe, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 18 publications below
• WB: See 88 publications below
• IHC: See 7 publications below
• IF: See 11 publications below
• IP: See 4 publications below
• CoIP: See 4 publications below

Produktinformation

15421-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA ACLY und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACLY fusion protein Ag7709
• Vollständiger Name: ATP citrate lyase
• Berechnetes Molekulargewicht: 121 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC006195
• Gene symbol: ACLY
• Gene ID (NCBI): 47
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	Nature; Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate.; Authors - Steven Zhao
mouse	WB,IF	Nature; Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.; Authors - Philipp Mews
mouse,human	WB	Cell; Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.; Authors - Xiaojing Liu ; KO Validated
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
mouse	WB	Mol Cell; Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation; Authors - Sophie Trefely ; KO Validated
human	WB	Mol Cell; Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.; Authors - Sharanya Sivanand ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Uthra (Verified Customer) (01-28-2021)

Excellent for IHC of the liver tissue under PFA fixation. The dilution of primary antibody I used 1:200.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Liver

ACLY Antibody Immunohistochemistry validation (1:200 dilution) in Liver (Cat no:15421-1-AP)

FH

K (Verified Customer) (12-20-2020)

This antibody is working good for WB (1:1000) and IHC (1:200)

• Applications: Western Blot, Immunohistochemistry

ACLY Antibody Western Blot,Immunohistochemistry validation ( dilution) in (Cat no:15421-1-AP)