ACMSD Rekombinanter Antikörper

ACMSD Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241077D5

Kat-Nr. : 84100-3-RR

Synonyme

EC:4.1.1.45, 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase, 241077D5

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  WB analysis of HEK-293 using 84100-3-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 84100-3-RR (ACMSD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 84100-3-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACMSD Recombinant antibody (84100-3-RR, Clone:241077D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84100-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84100-3-RR against Human ACMSD were performed. The affinity constant is 3.49 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84100-3-RR bindet in WB, FC (Intra), ELISA ACMSD und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ACMSD fusion protein Ag25212
• Vollständiger Name: aminocarboxymuconate semialdehyde decarboxylase
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC016018
• Gene symbol: ACMSD
• Gene ID (NCBI): 130013
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACMSD (alpha-amino-beta-carboxy-muconate-semialdehyde decarboxylase ) is the key enzyme that regulates the de novo NAD+ synthesis from tryptophan. ACMSD is highly expressed in the liver, kidney and to a lower degree in brain under physiological conditions. The enzyme is primarily cytosolic and is positioned at a critical branching point in the kynurenine pathway, wherein it influences the fate of the precursor 2-amino-3-carboxymuconic-6-semialdehyde (ACMS).