Aconitase 2 Polyklonaler Antikörper

Aconitase 2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Rezensionen (2)

Kat-Nr. : 11134-1-AP

Synonyme

ACO2, Aconitase, aconitase 2, aconitase 2, mitochondrial, ACONM, Citrate hydro lyase

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Galerie der Validierungsdaten

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  WB analysis using 11134-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11134-1-AP (Aconitase 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11134-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11134-1-AP (Aconitase 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11134-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11134-1-AP (Aconitase 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11134-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11134-1-AP (Aconitase 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11134-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11134-1-AP (Aconitase 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11134-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 11134-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11134-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of HeLa using 11134-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11134-1-AP (Aconitase 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HeLa-Zellen, humanes Herzgewebe, humanes Lebergewebe, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IF: See 1 publications below

Produktinformation

11134-1-AP bindet in WB, IHC, IF, ELISA Aconitase 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Aconitase 2 fusion protein Ag1590
• Vollständiger Name: aconitase 2, mitochondrial
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC014092
• Gene symbol: ACO2
• Gene ID (NCBI): 50
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).

Publikationen

Species	Application	Title
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
human	WB	Cell Rep; A LON-ClpP Proteolytic Axis Degrades Complex I to Extinguish ROS Production in Depolarized Mitochondria.; Authors - Kenneth Robert Pryde
human	WB	Cell Biosci; Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth; Authors - Hongting Zhao
human	WB	Mol Cell Proteomics; A combined proteomics and metabolomics profiling of gastric cardia cancer reveals characteristic dysregulations in glucose metabolism.; Authors - Cai Zhen Z
mouse	WB	J Nanobiotechnology; Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation.; Authors - Li Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Svitlana (Verified Customer) (03-19-2019)

SDS-PAGE: mitochondrial lysate 10 ug protein/well, 4-12% Bis-Tris.Transfer: Immobilon-FL transfer membranes O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1h at room temperature.Secondary Ab: IRDye 800CW Goat anti-Rabbit 1 h at room temperature.Lines on WB:1. BioRad Precision Plus Protein standard2. Lysate of mitochondrial fraction.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293t mitochondria

Aconitase 2 Antibody Western Blot, validation (1:1000 dilution) in HEK293t mitochondria (Cat no:11134-1-AP)