ACOT11 Polyklonaler Antikörper

ACOT11 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10776-1-PBS

Synonyme

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  WB analysis using 10776-1-AP (same clone as 10776-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10776-1-AP (ACOT11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10776-1-AP (same clone as 10776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10776-1-AP (ACOT11 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10776-1-AP (same clone as 10776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10776-1-AP (ACOT11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 10776-1-AP (same clone as 10776-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10776-1-AP (ACOT11 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 10776-1-AP (same clone as 10776-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ACOT11 antibody (10776-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10776-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ACOT11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACOT11 fusion protein Ag1176
• Vollständiger Name: acyl-CoA thioesterase 11
• Berechnetes Molekulargewicht: 68.4 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC001517
• Gene symbol: ACOT11
• Gene ID (NCBI): 26027
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACOT11(Acyl-coenzyme A thioesterase 11) is also named as BFIT, KIAA0707, THEA.BFIT is mapped to syntenic regions of chromosomes 1 (human) and 4 (mouse) associated with body fatness and diet-induced obesity, potentially linking a deficit of BFIT activity with exacerbation of these traits and supports the transition of this tissue towards increased metabolic activity, probably through alteration of intracellular fatty acyl-CoA concentration(PMID:11696000).