ACOT2 Rekombinanter Antikörper

ACOT2 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251453E1

Kat-Nr. : 82122-5-PBS

Synonyme

acyl CoA thioesterase 2, Acyl-CoA thioesterase 2, Acyl-coenzyme A thioester hydrolase 2a, Acyl-coenzyme A thioesterase 2, mitochondrial, CTE-Ia

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  WB analysis using 82122-5-RR (same clone as 82122-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82122-5-RR (ACOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82122-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 82122-5-RR (same clone as 82122-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82122-5-RR (ACOT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82122-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 82122-5-RR (same clone as 82122-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82122-5-RR (ACOT2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82122-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82122-5-RR (same clone as 82122-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACOT2 antibody (82122-5-RR, Clone: 251453E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82122-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82122-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ACOT2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ACOT2 fusion protein Ag8093
• Vollständiger Name: acyl-CoA thioesterase 2
• Berechnetes Molekulargewicht: 483 aa, 53 kDa
• Beobachtetes Molekulargewicht: 46-53 kDa
• GenBank-Zugangsnummer: BC006335
• Gene symbol: ACOT2
• Gene ID (NCBI): 10965
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acyl-CoA thioesterase (Acot)2 localizes to the mitochondrial matrix and hydrolyses long-chain fatty acyl-CoA into free FA and CoASH. Acot2 is expressed in highly oxi­dative tissues and is poised to modulate mitochondrial FA oxidation (FAO) (PMID: 25114170). The structure of ACOT2 consists of two domains, N and C domains, and the active site of ACOT2 is located at the interface between the N and C domains (PMID: 19497300).