ACOT7 Polyklonaler Antikörper

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• KD/KO Validated

ACOT7 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 15972-1-AP

Synonyme

ACH1, ACOT7, ACT, acyl CoA thioesterase 7, BACH, Brain acyl CoA hydrolase, CTE II, CTE IIa, hBACH, LACH, LACH1

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  WB analysis using 15972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15972-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15972-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15972-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, humanes Hirngewebe, Jurkat-Zellen, Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

15972-1-AP bindet in WB, IHC, IF, ELISA ACOT7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACOT7 fusion protein Ag8759
• Vollständiger Name: acyl-CoA thioesterase 7
• Berechnetes Molekulargewicht: 370 aa, 40 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC017365
• Gene symbol: ACOT7
• Gene ID (NCBI): 11332
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:10578051).  Acyl-coenzyme A thioesterase 7/ACOT7 preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed:10578051). May play an important physiological function in brain (PubMed:10578051). ACOT7 has 7 isoforms between 27-40 kDa, we can detect a band at 37 kDa. ACOT7 is high homology with ACOT7L, this antibody may also detect ACOT7L.

Publikationen

Species	Application	Title
		Nat Commun; Convergent genomic signatures of flight loss in birds suggest a switch of main fuel.; Authors - Shengkai Pan
human	WB,IHC	J Hepatocell Carcinoma; Acyl-CoA Thioesterase 7 is Transcriptionally Activated by Krüppel-Like Factor 13 and Promotes the Progression of Hepatocellular Carcinoma.; Authors - Xingming Xie
mouse	WB	J Proteomics; TMT-labeled quantitative malonylome analysis on the longissimus dorsi muscle of Laiwu pigs reveals the role of ACOT7 in fat deposition; Authors - Wenlei Wang
human	WB,IHC	Acta Histochem; ACOT7 positively regulated by CREB1 promotes the progression of cutaneous melanoma; Authors - Ni Tang
bovine	WB,IHC,IF	Int J Mol Sci; Role of Acyl-CoA Thioesterase 7 in Regulating Fatty Acid Metabolism and Its Contribution to the Onset and Progression of Bovine Clinical Mastitis; Authors - Bin Zhou
mouse	WB	In Vitro Cell Dev Biol Anim; ACOT7 protects epidermal stem cells against lipid peroxidation; Authors - Guang Zhang