ACOT9 Polyklonaler Antikörper

ACOT9 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 15901-1-AP

Synonyme

ACATE2, ACOT9, Acyl CoA thioester hydrolase 9, acyl CoA thioesterase 9, CGI 16, MT ACT48

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Galerie der Validierungsdaten

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  WB analysis using 15901-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACOT9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15901-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACTO9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15901-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACTO9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15901-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACTO9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15901-1-AP (ACOT9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15901-1-AP (ACOT9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of rat kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of rat kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausherzgewebe, Mausnierengewebe, MCF-7-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Nierengewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

15901-1-AP bindet in WB, IHC, ELISA ACOT9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACOT9 fusion protein Ag8730
• Vollständiger Name: acyl-CoA thioesterase 9
• Berechnetes Molekulargewicht: 24 kDa, 49 kDa
• Beobachtetes Molekulargewicht: 43-50 kDa
• GenBank-Zugangsnummer: BC012573
• Gene symbol: ACOT9
• Gene ID (NCBI): 23597
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACOT9 (Acyl-coenzyme A thioesterase 9) is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT9 has 4 isoforms with the molecular mass of 43-50 kDa.

Publikationen

Species	Application	Title
human	IHC	Liver Int; Acyl-CoA thioesterase 9 promotes tumor growth and metastasis through reprogramming of fatty acid metabolism in hepatocellular carcinoma; Authors - Bao Wang ; KD Validated
mouse	WB	EMBO Mol Med; A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy; Authors - Nneka Southwell