ACP5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ACP5 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human und mehr (2)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 11594-1-AP

Synonyme

ACP5, Tartrate resistant acid ATPase, TR AP, TRACP, TRAP, TrATPase, Type 5 acid phosphatase

---

Galerie der Validierungsdaten

• 

  WB analysis using 11594-1-AP

  pig tonsil tissue was subjected to SDS PAGE followed by western blot with 11594-1-AP (ACP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  FC experiment of A431 using 11594-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human ACP5 (11594-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: pig tonsil tissue
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Produktinformation

11594-1-AP bindet in WB, IHC, IF, FC, ELISA ACP5 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACP5 fusion protein Ag2065
• Vollständiger Name: acid phosphatase 5, tartrate resistant
• Berechnetes Molekulargewicht: 325 aa, 37 kDa
• GenBank-Zugangsnummer: BC025414
• Gene symbol: ACP5
• Gene ID (NCBI): 54
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACP5(acid phosphatase 5, tartrate resistant) is also named as TRAP, TrATPase and belongs to the metallophosphoesterase superfamily. It is an iron-containing glycoprotein with a molecular mass of approximately 34 kD. ACP5 is a histochemical marker of the osteoclast. It is also characteristic of monohistiocytes, particularly alveolar macrophages, and is associated with diverse pathological conditions, including hairy cell leukemia and AIDS encephalopathy.(PMID:10639488).

Publikationen

Species	Application	Title
human,mouse	IF	Nat Commun; Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.; Authors - Yinan Hu ; KO Validated
mouse		Redox Biol; GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux; Authors - Xiaoxiao Ji
mouse	IHC	Hypertension; Secreted Klotho Attenuates Inflammation-Associated Aortic Valve Fibrosis in Senescence-Accelerated Mice P1.; Authors - Jianglei Chen
mouse	IHC	Bioact Mater; Bisphosphonate-based hydrogel mediates biomimetic negative feedback regulation of osteoclastic activity to promote bone regeneration.; Authors - Zhuo Li
mouse		FASEB J; The function of the calcium channel Orai1 in osteoclast development.; Authors - Lisa J Robinson
human	IF	Front Oncol; Single-Cell RNA Sequencing Reveals the Migration of Osteoclasts in Giant Cell Tumor of Bone; Authors - Wenyu Feng