ACP5 Polyklonaler Antikörper

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• KD/KO Validated

ACP5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(30)

Kat-Nr. : 11594-1-AP

Synonyme

ACP 5, EC:3.1.3.2, Tartrate-resistant acid ATPase, Tartrate-resistant acid phosphatase type 5, TR-AP

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  WB analysis using 11594-1-AP

  pig tonsil tissue was subjected to SDS PAGE followed by western blot with 11594-1-AP (ACP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 11594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11594-1-AP (ACP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: pig tonsil tissue
• Erfolgreiche Detektion in IHC: Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Produktinformation

11594-1-AP bindet in WB, IHC, IF, ELISA ACP5 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACP5 fusion protein Ag2065
• Vollständiger Name: acid phosphatase 5, tartrate resistant
• Berechnetes Molekulargewicht: 325 aa, 37 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC025414
• Gene symbol: ACP5
• Gene ID (NCBI): 54
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACP5(acid phosphatase 5, tartrate resistant) is also named as TRAP, TrATPase and belongs to the metallophosphoesterase superfamily. It is an iron-containing glycoprotein with a molecular mass of approximately 34 kD. ACP5 is a histochemical marker of the osteoclast. It is also characteristic of monohistiocytes, particularly alveolar macrophages, and is associated with diverse pathological conditions, including hairy cell leukemia and AIDS encephalopathy.(PMID:10639488).

Publikationen

Species	Application	Title
mouse,human	IF	Nat Commun; Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.; Authors - Yinan Hu ; KO Validated
mouse	WB	Redox Biol; GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux; Authors - Xiaoxiao Ji
mouse	IHC	Hypertension; Secreted Klotho Attenuates Inflammation-Associated Aortic Valve Fibrosis in Senescence-Accelerated Mice P1.; Authors - Jianglei Chen
mouse	WB	ACS Appl Mater Interfaces; Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis; Authors - Wenzheng Liao
mouse	IHC	Bioact Mater; Bisphosphonate-based hydrogel mediates biomimetic negative feedback regulation of osteoclastic activity to promote bone regeneration.; Authors - Zhuo Li
mouse	WB	iScience; StemRegenin 1 attenuates the RANKL-induced osteoclastogenesis via inhibiting AhR-c-src-NF-κB/p-ERK MAPK-NFATc1 signaling pathway; Authors - Shengji Zhou