ACP6 Polyklonaler Antikörper

ACP6 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12965-1-AP

Synonyme

Acid phosphatase-like protein 1, ACPL1, EC:3.1.3.2, LPAP, PACPL1

---

• 

  WB analysis of human liver using 12965-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12965-1-AP (ACP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12965-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12965-1-AP (ACP6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12965-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12965-1-AP (ACP6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 12965-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12965-1-AP (ACP6 antibody) at dilution of 1:100 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, COLO 320-Zellen, HepG2-Zellen, humanes Lebergewebe, Mauslebergewebe, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12965-1-AP bindet in WB, IHC, ELISA ACP6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACP6 fusion protein Ag3633
• Vollständiger Name: acid phosphatase 6, lysophosphatidic
• Berechnetes Molekulargewicht: 428 aa, 49 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC034686
• Gene symbol: ACP6
• Gene ID (NCBI): 51205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lysophosphatidic acid phosphatase type 6 (ACP6) is an LPA-specific acid phosphatase that hydrolyzes LPA to monoacylglycerol (MAG) and phosphate. Previous studies have shown that ACP6 is localized in the mitochondria with ubiquitous expression throughout all tissues, and high expression levels in kidney, heart, small intestine, muscle, and liver. Through specifi cally hydrolyzing LPA, ACP6 regulates mitochondrial lipid biosynthesis, thereby controlling important mitochondrial functions. It was also shown that ACP6 might be involved in tumor progression, suggesting that ACP6 could be a good candidate marker for the prognosis of esophageal cancer. Uniprot showed that by alternative splicing, ACP6 may have two kinds of shear fragments with molecular weights of 49 and 31kDa respectively. (PMID: 23807634)