ACSF2 Polyklonaler Antikörper

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• KD/KO Validated

ACSF2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 16140-1-AP

Synonyme

UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2

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  WB analysis of mouse lung using 16140-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16140-1-AP (ACSF2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human colon cancer using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16140-1-AP (ACSF2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16140-1-AP (ACSF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16140-1-AP (ACSF2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16140-1-AP (ACSF2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 16140-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 16140-1-AP (ACSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

16140-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA ACSF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACSF2 fusion protein Ag9199
• Vollständiger Name: acyl-CoA synthetase family member 2
• Berechnetes Molekulargewicht: 615 aa, 68 kDa
• Beobachtetes Molekulargewicht: 55-70 kDa
• GenBank-Zugangsnummer: BC012053
• Gene symbol: ACSF2
• Gene ID (NCBI): 80221
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.

Publikationen

Species	Application	Title
rat	WB,IP	Prostaglandins Other Lipid Mediat; Targeted Lipidomics Analysis of Lysine 179 Acetylation of ACSF2 in Rat Hepatic Stellate Cells; Authors - Meili Wang
mouse,rat	WB,IHC	Life Sci; ACSF2-mediated ferroptosis is involved in ulcerative colitis; Authors - Lianxiang Luo
human	WB,IF	Free Radic Biol Med; Inhibition of ACSF2 protects against renal ischemia/reperfusion injury via mediating mitophagy in proximal tubular cells; Authors - Haoyu Shi ; KD Validated
human	IHC	Clin Cancer Res; Biomarker identification and risk prediction model development for differentiated thyroid carcinoma lung metastasis based on primary lesion proteomics; Authors - Xiaoqi Peng
human,mouse	WB,IHC,IF	Diabetologia; ACSF2 and lysine lactylation contribute to renal tubule injury in diabetes; Authors - Jingfang Chen
human	WB,CoIP	Mol Carcinog; M2 macrophage exosome-derived Apoc1 promotes ferroptosis resistance in osteosarcoma by inhibiting ACSF2 deubiquitination; Authors - Ping Yin