ACSF2 Rekombinanter Antikörper

ACSF2 Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241208D9

Kat-Nr. : 84033-3-PBS

Synonyme

241208D9, ACSF 2, EC:6.2.1.2, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, UNQ493/PRO1009

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  WB analysis using 84033-3-RR (same clone as 84033-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84033-3-RR (ACSF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84033-3-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 84033-3-RR (same clone as 84033-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug ACSF2 Recombinant antibody (84033-3-RR, Clone:241208D9) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84033-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00952-1

  Cytometric bead array standard curve of MP00952-1, ACSF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84033-2-PBS. Detection antibody: 84033-3-PBS. Standard: Ag9199. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00952-3

  Cytometric bead array standard curve of MP00952-3, ACSF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84033-1-PBS. Detection antibody: 84033-3-PBS. Standard: Ag9199. Range: 2.5-80 ng/mL.

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  Affinity and Kinetic Characterization of 84033-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 84033-3-PBS against Human ACSF2 was performed. The affinity constant is below 1 pM.

• 

  Affinity and Kinetic Characterization of 84033-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84033-3-RR against Human ACSF2 were performed. The affinity constant is 1.94 nM.

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Geprüfte Anwendungen

Produktinformation

84033-3-PBS bindet in WB, FC (Intra), Cytometric bead array, Indirect ELISA ACSF2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ACSF2 fusion protein Ag9199
• Vollständiger Name: acyl-CoA synthetase family member 2
• Berechnetes Molekulargewicht: 615 aa, 68 kDa
• Beobachtetes Molekulargewicht: 51-71 kDa
• GenBank-Zugangsnummer: BC012053
• Gene symbol: ACSF2
• Gene ID (NCBI): 80221
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.