ACSL3 Polyklonaler Antikörper

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• KD/KO Validated

ACSL3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20710-1-PBS

Synonyme

EC:6.2.1.3, EC:6.2.1.2, EC:6.2.1.15, Arachidonate--CoA ligase, ACSL 3

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  WB analysis of HEK-293 using 20710-1-AP (same clone as 20710-1-PBS)

  WB result of ACSL3 antibody (20710-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL3 transfected HEK-293 cells. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

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  WB analysis using 20710-1-AP (same clone as 20710-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20710-1-AP (ACSL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 20710-1-AP (same clone as 20710-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 20710-1-AP (ACSL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20710-1-AP (same clone as 20710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20710-1-AP (ACSL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20710-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20710-1-PBS bindet in WB, IHC, Indirect ELISA ACSL3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: acyl-CoA synthetase long-chain family member 3
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: NM_004457
• Gene symbol: ACSL3
• Gene ID (NCBI): 2181
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.