ACSL4/FACL4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ACSL4/FACL4 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(308)

Rezensionen (1)

Kat-Nr. : 22401-1-AP

Synonyme

ACSL4, FACL4, ACS4, ACSL 4, Arachidonate--CoA ligase

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  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22401-1-AP

  WB result of ACSL4/FACL4 antibody (22401-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4/FACL4 transfected HeLa cells.

• 

  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22401-1-AP

  IP result of anti-ACSL4/FACL4 (IP:22401-1-AP, 4ug; Detection:22401-1-AP 1:5000) with HEK-293 cells lysate 1400 ug.

• 

  IHC staining of mouse brain using 22401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Mausnierengewebe, NIH/3T3-Zellen, Rattennierengewebe, RAW 264.7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 272 publications below
• IHC: See 54 publications below
• IF: See 32 publications below
• IP: See 5 publications below
• CoIP: See 3 publications below

Produktinformation

22401-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA ACSL4/FACL4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACSL4/FACL4 fusion protein Ag18085
• Vollständiger Name: acyl-CoA synthetase long-chain family member 4
• Berechnetes Molekulargewicht: 711 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa, 74 kDa
• GenBank-Zugangsnummer: BC034959
• Gene symbol: ACSL4
• Gene ID (NCBI): 2182
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).

Publikationen

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
human	CoIP	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding
rat	WB,IHC	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
human	WB	Adv Sci (Weinh); Sirtuin1 Suppresses Calcium Oxalate Nephropathy via Inhibition of Renal Proximal Tubular Cell Ferroptosis Through PGC-1α-mediated Transcriptional Coactivation; Authors - Chen Duan
human	WB	Adv Sci (Weinh); Development and Application of the CRISPR-dcas13d-eIF4G Translational Regulatory System to Inhibit Ferroptosis in Calcium Oxalate Crystal-Induced Kidney Injury; Authors - Ziqi He

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

YINGJIAN (Verified Customer) (06-24-2025)

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: H9C2 cells

ACSL4/FACL4 Antibody Western Blot validation (1:5000 dilution) in H9C2 cells (Cat no:22401-1-AP)