ACSL4/FACL4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ACSL4/FACL4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

89

Kat-Nr. : 22401-1-AP

Synonyme

ACS4, ACSL4, ACSL4/FACL4, FACL4, LACS 4, LACS4, MRX63, MRX68

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Galerie der Validierungsdaten

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  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22401-1-AP

  WB result of ACSL4/FACL4 antibody (22401-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4/FACL4 transfected HeLa cells.

• 

  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22401-1-AP (ACSL4/FACL4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22401-1-AP

  IP result of anti-ACSL4/FACL4(IP:22401-1-AP, 4ug; Detection:22401-1-AP 1:5000) with HEK-293 cells lysate 1400 ug.

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  IHC staining of human placenta using 22401-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 22401-1-AP (ACSL4/FACL4 antibody at dilution of 1:50.

• 

  IHC staining of human placenta using 22401-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 22401-1-AP (ACSL4/FACL4 antibody at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 77 publications below
• IHC: See 17 publications below
• IF: See 6 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

22401-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ACSL4/FACL4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACSL4/FACL4 fusion protein Ag18085
• Vollständiger Name: acyl-CoA synthetase long-chain family member 4
• Berechnetes Molekulargewicht: 711 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa, 74 kDa
• GenBank-Zugangsnummer: BC034959
• Gene symbol: ACSL4
• Gene ID (NCBI): 2182
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).

Publikationen

Species	Application	Title
human	CoIP	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding
human	WB	Mol Ther; STING/ACSL4 axis-dependent ferroptosis and inflammation promote hypertension-associated chronic kidney disease; Authors - Li Gao
human	WB	EMBO J; FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.; Authors - Wenxian Wu
human	WB	Redox Biol; Non-enzymatic lipid peroxidation initiated by photodynamic therapy drives a distinct ferroptosis-like cell death pathway.; Authors - Sufang Shui
human	WB,IF	Redox Biol; Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.; Authors - Caiying Liu