ACSL4/FACL4 Monoklonaler Antikörper

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ACSL4/FACL4 Monoklonal Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H5D3

Kat-Nr. : 66617-1-PBS

Synonyme

ACSL4, FACL4, 1H5D3, ACS4, ACSL 4

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  WB analysis using 66617-1-Ig (same clone as 66617-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66617-1-Ig (same clone as 66617-1-PBS)

  WB result of ACSL4 antibody (66617-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66617-1-Ig (same clone as 66617-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66617-1-Ig (same clone as 66617-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 66617-1-Ig (same clone as 66617-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66617-1-Ig (same clone as 66617-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66617-1-Ig (same clone as 66617-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66617-1-Ig (same clone as 66617-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66617-1-Ig (same clone as 66617-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 66617-1-Ig (same clone as 66617-1-PBS)

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human ACSL4 (66617-1-Ig, Clone:1H5D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66617-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA ACSL4/FACL4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ACSL4/FACL4 fusion protein Ag18085
• Vollständiger Name: acyl-CoA synthetase long-chain family member 4
• Berechnetes Molekulargewicht: 711 aa, 79 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC034959
• Gene symbol: ACSL4
• Gene ID (NCBI): 2182
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.