ACSM5 Polyklonaler Antikörper

ACSM5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16591-1-AP

Synonyme

MACS3, EC:6.2.1.2, Acyl-coenzyme A synthetase ACSM5, mitochondrial, Acyl-CoA synthetase medium-chain family member 5, ACSM 5

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  WB analysis using 16591-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16591-1-AP (ACSM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16591-1-AP (ACSM5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse liver using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16591-1-AP (ACSM5 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 16591-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16591-1-AP (ACSM5 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16591-1-AP bindet in WB, IHC, ELISA ACSM5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACSM5 fusion protein Ag9373
• Vollständiger Name: acyl-CoA synthetase medium-chain family member 5
• Berechnetes Molekulargewicht: 208 aa, 23 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC016703
• Gene symbol: ACSM5
• Gene ID (NCBI): 54988
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	Mol Metab; Calponin 2 harnesses metabolic reprogramming to determine kidney fibrosis; Authors - Yuan Gui