ACSS2 Polyklonaler Antikörper

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• KD/KO Validated

ACSS2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Rezensionen (1)

Kat-Nr. : 16087-1-AP

Synonyme

ACAS2, AceCS1, Acetate--CoA ligase, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1

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  WB analysis using 16087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16087-1-AP

  IP result of anti-ACSS2 (IP:16087-1-AP, 4ug; Detection:16087-1-AP 1:500) with HepG2 cells lysate 5600 ug.

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  IHC staining of human brain using 16087-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 16087-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 synthetase antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Maushirngewebe, Mauslebergewebe, Neuro-2a-Zellen, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Produktinformation

16087-1-AP bindet in WB, IHC, IF, IP, ELISA ACSS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACSS2 fusion protein Ag9121
• Vollständiger Name: acyl-CoA synthetase short-chain family member 2
• Berechnetes Molekulargewicht: 701 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC010141
• Gene symbol: ACSS2
• Gene ID (NCBI): 55902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714). ACSS2 has two isoforms with the molecular weight of 78 and 80 kDa.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
human,mouse	WB,IHC,IF	Nat Commun; ACSS2 protects against alcohol-induced hepatocyte ferroptosis through regulation of hepcidin expression; Authors - Mengyao Wang
mouse	WB	Mol Cell; Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.; Authors - Anyuan He
	WB	Plant Physiol; FDX5 deletion affects metabolism of algae during the different phases of S-deprivation.; Authors - Venkataramanan Subramanian
mouse	WB	Antioxidants (Basel); Bisdemethoxycurcumin Alleviates Dextran Sodium Sulfate-Induced Colitis via Inhibiting NLRP3 Inflammasome Activation and Modulating the Gut Microbiota in Mice; Authors - Jingfei Zhang
mouse	WB,IF	Am J Physiol Gastrointest Liver Physiol; N-glycosylation of SCAP exacerbates hepatocellular inflammation and lipid accumulation via ACSS2-mediated histone H3K27 acetylation; Authors - Xuemei Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kishor (Verified Customer) (01-30-2019)

Good results in western blotting at 1:1000 dilution for human cell lines and rat liver tissue.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue