ACSS2 Polyklonaler Antikörper

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• KD/KO Validated

ACSS2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16087-1-PBS

Synonyme

ACAS2, AceCS1, Acetate--CoA ligase, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1

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  WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16087-1-AP (same clone as 16087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16087-1-AP (ACSS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 16087-1-AP (same clone as 16087-1-PBS)

  IP result of anti-ACSS2 (IP:16087-1-AP, 4ug; Detection:16087-1-AP 1:500) with HepG2 cells lysate 5600 ug. This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16087-1-AP (same clone as 16087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16087-1-AP (ACSS2 synthetase antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16087-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16087-1-PBS bindet in WB, IHC, IP, Indirect ELISA ACSS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACSS2 fusion protein Ag9121
• Vollständiger Name: acyl-CoA synthetase short-chain family member 2
• Berechnetes Molekulargewicht: 701 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC010141
• Gene symbol: ACSS2
• Gene ID (NCBI): 55902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714). ACSS2 has two isoforms with the molecular weight of 78 and 80 kDa.