ACSS3 Polyklonaler Antikörper

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• KD/KO Validated

ACSS3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 16204-1-AP

Synonyme

EC:6.2.1.17, EC:6.2.1.1, Acyl-CoA synthetase short-chain family member 3, mitochondrial, Acyl-CoA synthetase short-chain family member 3, ACSS 3

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  WB analysis using 16204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16204-1-AP (ACSS3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16204-1-AP

  WB result of ACSS3 antibody (16204-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSS3 transfected HepG2 cells.

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  IHC staining of human liver using 16204-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16204-1-AP (ACSS3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16204-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16204-1-AP (ACSS3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of U2OS using 16204-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ACSS3 antibody (16204-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of U2OS using 16204-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ACSS3 antibody (16204-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 2 publications below

Produktinformation

16204-1-AP bindet in WB, IHC, IF/ICC, ELISA ACSS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACSS3 fusion protein Ag9173
• Vollständiger Name: acyl-CoA synthetase short-chain family member 3
• Berechnetes Molekulargewicht: 686 aa, 75 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC009317
• Gene symbol: ACSS3
• Gene ID (NCBI): 79611
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACSS3(acyl-CoA synthetase short-chain family member 3, mitochondrial) belongs to the ATP-dependent AMP-binding enzyme family. It activates acetate so that it can be used for lipid synthesis or for energy generation. The deduced 686-amino acid protein contains 4 of 5 motifs characteristic of acyl-CoA synthetases. This protein has 2 isoforms produced by alternative splicing. The full length protein has a transit peptide with 29 amino acids.

Publikationen

Species	Application	Title
	WB	Cell Metab; Amelioration of nonalcoholic fatty liver disease by inhibiting the deubiquitylating enzyme RPN11; Authors - Bing Zhou
human,mouse	WB,IHC	Cancer Commun (Lond); Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma; Authors - Xiaoteng Cui
	WB	Nat Commun; ACSS2 protects against alcohol-induced hepatocyte ferroptosis through regulation of hepcidin expression; Authors - Mengyao Wang
mouse	WB	Nutrients; Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent.; Authors - Karolin Weitkunat
human,mouse	WB	Biochem J; NRF2/ACSS2 axis mediates the metabolic effect of alcohol drinking on esophageal squamous cell carcinoma.; Authors - Joab Otieno Odera
human	WB	Cancer Genet; Prognosis prediction and drug guidance of ovarian serous cystadenocarcinoma through mitochondria gene-based model; Authors - Dongsheng Shen