ACTA1-Specific Polyklonaler Antikörper

ACTA1-Specific Polyklonal Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17521-1-PBS

Synonyme

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  WB analysis of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hysteromyoma using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hysteromyoma using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  FC experiment of C2C12 using 17521-1-AP (same clone as 17521-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTA1-Specific (17521-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17521-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA ACTA1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: actin, alpha 1, skeletal muscle
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: NM_001100
• Gene symbol: ACTA1
• Gene ID (NCBI): 58
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.