ACTA1-Specific Rekombinanter Antikörper

ACTA1-Specific Rekombinant Antikörper für WB, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, Huhn, human, Maus, Ratte

Anwendung

WB, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5B15

Kat-Nr. : 82714-5-PBS

Synonyme

ACTA1, Alpha Actin, α actin, Actin, alpha skeletal muscle, Actin alpha skeletal muscle

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  WB analysis using 82714-5-RR (same clone as 82714-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82714-5-RR (ACTA1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82714-5-PBS bindet in WB, IF-P, Indirect ELISA ACTA1-Specific und zeigt Reaktivität mit Hausschwein, Huhn, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: actin, alpha 1, skeletal muscle
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: NM_001100
• Gene symbol: ACTA1
• Gene ID (NCBI): 58
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.