beta actin Polyklonaler Antikörper

beta actin Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10230-1-AP

Synonyme

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

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Galerie der Validierungsdaten

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Geprüfte Anwendungen

Empfohlene Verdünnung

Anwendung	Verdünnung
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below

Produktinformation

10230-1-AP bindet in WB, ELISA beta actin und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: beta actin fusion protein Ag0297
• Vollständiger Name: actin, beta
• Berechnetes Molekulargewicht: 375 aa, 42 kDa
• GenBank-Zugangsnummer: BC002409
• Gene symbol: ACTB
• Gene ID (NCBI): 60
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Beta Actin has been widely used as the internal control in bioscience. At least six isoforms are known in mammals. ALPHA-actin was predominant in single cells and BETA-actin was major in the cultured cells. Nonmuscle BETA- and GAMMA-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells. ALPHA-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, ALPHA- and GAMMA-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. This antibody can detect endogenous level of β-Actin and may cross-react with other isoforms of actin family.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.

Publikationen

Species	Application	Title
human	WB	Int J Nanomedicine; Integration of Dual Targeting and Dual Therapeutic Modules Endows Self-Assembled Nanoparticles with Anti-Tumor Growth and Metastasis Functions.; Authors - Biao Chen
human	WB	J Cell Biochem; Dependence of artesunate on long noncoding RNA-RP11 to inhibit epithelial-mesenchymal transition of hepatocellular carcinoma.; Authors - Wu Jing
bovine	WB	Biol Trace Elem Res; The Protective Effect of Selenium on Bovine Mammary Epithelial Cell Injury Caused by Depression of Thioredoxin Reductase.; Authors - Yongmei Guo
human	WB	BMC Urol; Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway.; Authors - Cai Lv
	WB	Int J Mol Med; miR‑16‑2‑3p inhibits cell proliferation and migration and induces apoptosis by targeting PDPK1 in maxillary primordium mesenchymal cells.; Authors - Tao Han
human	WB	Int J Biol Macromol; Scavenger receptor a mediates glycated LDL transcytosis across endothelial cells to promote atherosclerosis; Authors - Wenzhuo Cheng