beta actin Monoklonaler Antikörper
beta actin Monoklonal Antikörper für ELISA
Wirt / Isotyp
Maus / IgM
Getestete Reaktivität
human und mehr (3)
Anwendung
WB, IP, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
7A9H3
Kat-Nr. : 60008-2-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 27 publications below |
IF | See 1 publications below |
IP | See 2 publications below |
Produktinformation
60008-2-Ig bindet in WB, IP, IF, ELISA beta actin und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human, Maus, Ratte, Rind |
Wirt / Isotyp | Maus / IgM |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | beta actin fusion protein Ag0297 |
Vollständiger Name | actin, beta |
Berechnetes Molekulargewicht | 375 aa, 42 kDa |
Beobachtetes Molekulargewicht | 45 kDa |
GenBank-Zugangsnummer | BC002409 |
Gene symbol | ACTB |
Gene ID (NCBI) | 60 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Caprylsäure/Ammoniumsulfat-Präzipitation |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins.
Publikationen
Species | Application | Title |
---|---|---|
Nat Commun The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression. | ||
Elife Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors. | ||
Bioeng Transl Med Neonatal microglia and proteinase inhibitors-treated adult microglia improve traumatic brain injury in rats by resolving the neuroinflammation. | ||
PLoS Genet Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing. |