Alpha Actin Polyklonaler Antikörper

Alpha Actin Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11032-1-AP

Synonyme

ACTC, ACTC1, ACTC1 cardiac muscle 1, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha actin, Alpha cardiac actin, CMD1R, CMH11

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 11032-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11032-1-AP (alpha Actin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11032-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11032-1-AP (alpha Actin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11032-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11032-1-AP (alpha Actin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11032-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11032-1-AP (alpha Actin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 11032-1-AP

  IP Result of anti-alpha Actin (IP:11032-1-AP, 4ug; Detection:11032-1-AP 1:800) with mouse heart tissue lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maus-Skelettmuskelgewebe, Maus-Thymusgewebe
• Erfolgreiche IP: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

11032-1-AP bindet in WB, IP, IHC, ELISA Alpha Actin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha Actin fusion protein Ag1471
• Vollständiger Name: actin, alpha, cardiac muscle 1
• Berechnetes Molekulargewicht: 377 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC009978
• Gene symbol: ACTC1
• Gene ID (NCBI): 70
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	FASEB J; Zbtb20 deficiency causes cardiac contractile dysfunction in mice.; Authors - An-Jing Ren
human	WB	Int J Biol Sci; MnSOD Lysine 68 acetylation leads to cisplatin and doxorubicin resistance due to aberrant mitochondrial metabolism.; Authors - Yucheng Gao
human	IHC	Stem Cells Dev; A model of early human embryonic stem cell differentiation reveals inter- and intracellular changes on transition to squamous epithelium.; Authors - Galat Vasiliy V
human	WB	Onco Targets Ther; Clinical Significance and Oncogenic Activity of GRWD1 Overexpression in the Development of Colon Carcinoma.; Authors - Xin Zhou