Alpha cardiac muscle actin specific Monoklonaler Antikörper

Alpha cardiac muscle actin specific Monoklonal Antikörper für WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F2B9

Kat-Nr. : 66125-1-PBS

Synonyme

ACTC1-specific, ACTC1, Alpha actin, 1F2B9, ACTC

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  WB analysis using 66125-1-Ig (same clone as 66125-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66125-1-Ig (ACTC1-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66125-1-Ig (ACTC1-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart and human skeletal muscle tissue using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart and human skeletal muscle using 66125-1-Ig (ACTC1 specific antibody) at dilution 1:500. (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

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  FC experiment of C2C12 using 66125-1-Ig (same clone as 66125-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTC1-specific (66125-1-Ig, Clone:1F2B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66125-1-PBS bindet in WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA Alpha cardiac muscle actin specific und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: actin, alpha, cardiac muscle 1
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: NM_005159
• Gene symbol: ACTC1
• Gene ID (NCBI): 70
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.