ACTG2 Polyklonaler Antikörper

ACTG2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31849-1-AP

Synonyme

ACTA3, Actin, gamma-enteric smooth muscle, Actin, gamma-enteric smooth muscle, intermediate form, ACTL3, ACTSG

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  WB analysis using 31849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31849-1-AP (ACTG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 31849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31849-1-AP (ACTG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 31849-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31849-1-AP (ACTG2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 31849-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31849-1-AP (ACTG2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maus-Skelettmuskelgewebe, Maus-Magengewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31849-1-AP bindet in WB, IHC, ELISA ACTG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACTG2 fusion protein Ag36024
• Vollständiger Name: actin, gamma 2, smooth muscle, enteric
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC094877
• Gene symbol: ACTG2
• Gene ID (NCBI): 72
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Actin gamma 2 (ACTG2, also known as ACT, ACTE, VSCM, ACTA3, ACTL3, and ACTSG) is a highly conserved protein involved in various types of cell motility and ubiquitously expressed in all eukaryotic cells. ACTG2 mutations would cause congenital distended bladder, microcolon, and intestinal hypoperistalsis (PMID: 24337657). It also has been reported that ACTG2 promotes hepatocellular carcinoma (HCC) migration and invasion through the Notch1 signaling pathway, which might serve as a new intervention target for the treatment of HCC (PMID: 28385530).