ACTL7A Polyklonaler Antikörper

ACTL7A Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 17355-1-AP

Synonyme

Actin like 7 alpha, actin like 7A, Actin like protein 7A, ACTL7A

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Galerie der Validierungsdaten

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  WB analysis using 17355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17355-1-AP (ACTL7A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 17355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17355-1-AP (ACTL7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 17355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17355-1-AP (ACTL7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17355-1-AP

  Immunofluorescent analysis of HepG2 cells, using ACTL7A antibody 17355-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Produktinformation

17355-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ACTL7A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACTL7A fusion protein Ag10613
• Vollständiger Name: actin-like 7A
• Berechnetes Molekulargewicht: 435 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC014610
• Gene symbol: ACTL7A
• Gene ID (NCBI): 10881
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACTL7a is located in the acrosome and tail of mature spermatozoa and plays an important role in spermiogenesis. Recently mutation of ACTL7A has been linked to infertility.

Publikationen

Species	Application	Title
human	IF,CoIP	Am J Hum Genet; Homozygous pathogenic variants in ACTL9 cause fertilization failure and male infertility in humans and mice.; Authors - Jing Dai
human	WB,IF	Hum Reprod; ACROSIN deficiency causes total fertilization failure in humans by preventing the sperm from penetrating the zona pellucida; Authors - Rong Hua
human	CoIP	Hum Reprod; Loss of SPACA1 function causes autosomal recessive globozoospermia by damaging the acrosome-acroplaxome complex.; Authors - Pingping Chen
human	WB,IF	Hum Reprod; Novel bi-allelic variants in ACTL7A are associated with male infertility and total fertilization failure.; Authors - Jing Wang
human,mouse	WB,IF,IP	Development; Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice.; Authors - Xiao-Zhen Zhang
human,mouse	WB,IF,IP	Mol Hum Reprod; Pathogenic variant in ACTL7A causes severe teratozoospermia characterized by bubble-shaped acrosomes and male infertility.; Authors - Jing Dai