ACTN2 Rekombinanter Antikörper

ACTN2 Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250229F6

Kat-Nr. : 85857-4-PBS

Synonyme

Actinin alpha 2, actinin, alpha 2, ACTN 2, Alpha actinin 2, Alpha-actinin skeletal muscle isoform 2

---

• 

  WB analysis using 85857-4-RR (same clone as 85857-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85857-4-RR (ACTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 85857-4-RR (same clone as 85857-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85857-4-RR (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 85857-4-RR (same clone as 85857-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 85857-4-RR (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

• 

  IF Staining of H9C2 using 85857-4-RR (same clone as 85857-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using ACTN2 antibody (85857-4-RR, Clone: 250229F6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85857-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP02153-1

  Cytometric bead array standard curve of MP02153-1, ACTN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85857-4-PBS. Detection antibody: 85857-3-PBS. Standard: Ag5459. Range: 0.781-100 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

85857-4-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA ACTN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ACTN2 fusion protein Ag5459
• Vollständiger Name: actinin, alpha 2
• Berechnetes Molekulargewicht: 104 kDa
• Beobachtetes Molekulargewicht: 103 kDa
• GenBank-Zugangsnummer: BC051770
• Gene symbol: ACTN2
• Gene ID (NCBI): 88
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles, and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).