ACTN2 Polyklonaler Antikörper

ACTN2 Polyklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-14221

Synonyme

Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, Alpha actinin 2, ACTN 2, actinin, alpha 2

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  IF Staining of mouse heart using CL488-14221

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite® Plus 488 ACTN2 antibody (CL488-14221) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of H9C2 using CL488-14221

  Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using CoraLite® Plus 488 ACTN2 antibody (CL488-14221) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: H9C2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-14221 bindet in IF/ICC, IF-P ACTN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACTN2 fusion protein Ag5459
• Vollständiger Name: actinin, alpha 2
• Berechnetes Molekulargewicht: 104 kDa
• Beobachtetes Molekulargewicht: 103 kDa
• GenBank-Zugangsnummer: BC051770
• Gene symbol: ACTN2
• Gene ID (NCBI): 88
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).