ACTN3 Polyklonaler Antikörper

ACTN3 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (2)

Kat-Nr. : 24378-1-AP

Synonyme

actinin, alpha 3, ACTN 3, Alpha actinin 3, Alpha-actinin skeletal muscle isoform 3, Alpha-actinin-3

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  WB analysis of mouse skeletal muscle using 24378-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24378-1-AP (ACTN3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 24378-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24378-1-AP (ACTN3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 24378-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 24378-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24378-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24378-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 24378-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (24378-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 24378-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (24378-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

24378-1-AP bindet in WB, IHC, IF-P, ELISA ACTN3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACTN3 fusion protein Ag19479
• Vollständiger Name: actinin, alpha 3
• Berechnetes Molekulargewicht: 901 aa, 103 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC099647
• Gene symbol: ACTN3
• Gene ID (NCBI): 89
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Dokl Biochem Biophys; Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?; Authors - I O Petrova
mouse	WB	FASEB J; Overexpression of enhanced yellow fluorescent protein fused with Channelrhodopsin-2 causes contractile dysfunction in skeletal muscle; Authors - Syeda N Lamia
mouse	WB	Cell Metab; Muscle-derived small extracellular vesicles induce liver fibrosis during overtraining; Authors - Ya Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Edma (Verified Customer) (03-03-2025)

The antibody worked very well in western blot :)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549 cells

ACTN3 Antibody Western Blot validation (1:1000 dilution) in A549 cells (Cat no:24378-1-AP)

FH

Joleen (Verified Customer) (06-12-2019)

The antibody recognizes ACTN3 (top band) but also other non-specific proteins, though less prominently.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

ACTN3 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:24378-1-AP)