ACTN3 Rekombinanter Antikörper

ACTN3 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241168A5

Kat-Nr. : 84356-1-PBS

Synonyme

Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, Alpha actinin 3, ACTN 3, actinin, alpha 3

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  WB analysis of rat skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84356-1-RR (ACTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84356-1-RR (ACTN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (84356-1-RR, Clone: 241168A5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84356-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84356-1-RR against Human ACTN3 were performed. The affinity constant is 0.116 nM.

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Geprüfte Anwendungen

Produktinformation

84356-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA ACTN3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ACTN3 fusion protein Ag36709
• Vollständiger Name: actinin, alpha 3
• Berechnetes Molekulargewicht: 901 aa, 103 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC099647
• Gene symbol: ACTN3
• Gene ID (NCBI): 89
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.