ACTN4 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

ACTN4 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1E12B8

Kat-Nr. : 66628-1-Ig

Synonyme

ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4

---

Galerie der Validierungsdaten

• 

  WB analysis using 66628-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66628-1-Ig (ACTN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66628-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66628-1-Ig (ACTN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66628-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66628-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66628-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66628-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, Hausschwein-Hirngewebe, HEK-293-Zellen, Hühnerhirngewebe, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, Maushirngewebe, MCF-7-Zellen, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

66628-1-Ig bindet in WB, IP, IHC, IF, ELISA ACTN4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ACTN4 fusion protein Ag13549
• Vollständiger Name: actinin, alpha 4
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 105 and 80 kDa
• GenBank-Zugangsnummer: BC005033
• Gene symbol: ACTN4
• Gene ID (NCBI): 81
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 105 and 80 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)

Publikationen

Species	Application	Title
human	IF	Int Immunopharmacol; 1β-Hydroxyalantolactone from Inulae Flos alleviated the progression of pulmonary fibrosis via inhibiting JNK/FOXO1/NF-κB pathway; Authors - Bing Yu
human	WB	Environ Toxicol; TRIP13 exerts a cancer-promoting role in cervical cancer by enhancing Wnt/β-catenin signaling via ACTN4; Authors - Xiaoying Liu
	WB,IF	J Physiol Biochem; Dysregulated expression of ACTN4 contributes to endothelial cell injury via the activation of the p38-MAPK/p53 apoptosis pathway in preeclampsia.; Authors - Jianlin Zhao ; KD Validated
human	WB,IF,IP	Front Oncol; The deubiquitinating enzyme MINDY2 promotes pancreatic cancer proliferation and metastasis by stabilizing ACTN4 expression and activating the PI3K/AKT/mTOR signaling pathway; Authors - Peng Liu