ACTR10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ACTR10 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 20101-1-AP

Synonyme

ARP10, ACTR11, Actin-related protein 11, Actin-related protein 10, Actin related protein 11

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  WB analysis of mouse brain using 20101-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20101-1-AP (ACTR10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20101-1-AP

  IP result of anti-ACTR10 (IP:20101-1-AP, 4ug; Detection:20101-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human hepatocirrhosis using 20101-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20101-1-AP (ACTR10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20101-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20101-1-AP (ACTR10 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 20101-1-AP

  Immunofluorescent analysis of HepG2 cells, using ACTR10 antibody 20101-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

20101-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ACTR10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACTR10 fusion protein Ag13612
• Vollständiger Name: actin-related protein 10 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 417 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC011997
• Gene symbol: ACTR10
• Gene ID (NCBI): 55860
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Proc Natl Acad Sci U S A; Dynein/dynactin is necessary for anterograde transport of Mbp mRNA in oligodendrocytes and for myelination in vivo.; Authors - Amy L Herbert
human	WB	Curr Biol; An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation.; Authors - Florencia di Pietro
Monkey	WB	Mol Biol Cell; Dynactin's pointed-end complex is a cargo-targeting module.; Authors - Ting-Yu Yeh ; KD Validated
mouse	WB	Neurobiol Aging; Dynactin 6 deficiency enhances aging-associated dystrophic neurite formation in mouse brains.; Authors - Md Golam Sharoar

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zakia (Verified Customer) (05-22-2024)

we are using this for our lab, Works great,

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: N2A,293T cells, ReNcells

ACTR10 Antibody Western Blot,Immunohistochemistry validation (1:1000 dilution) in N2A,293T cells, ReNcells (Cat no:20101-1-AP)